In case of dead animals, the specimens shluld be taken soon after seath to avoid the chances of invasion of tissues by putrefactive bacteria rendering the specimens unsuitable for examination.
Однажды вечером на университетском представлении «Щелкунчика» Сьюзан предложила Дэвиду вскрыть шифр, который можно было отнести к числу базовых. Весь антракт он просидел с ручкой в руке, ломая голову над посланием из одиннадцати букв: HL FKZC VD LDS В конце концов, когда уже гасли огни перед началом второго акта, его осенило. Шифруя послание, Сьюзан просто заменила в нем каждую букву на предшествующую ей алфавите.
Для расшифровки Беккеру нужно было всего лишь подставить вместо имеющихся букв те, что следовали непосредственно за ними: А превращалось в В, В - в С и так далее.
Generous blocks of fresh tissues such as liver, spleen, kidneys, lymph nodes, lungs and brain may be forwarded refrigerated but not frozen in wide mouth sterile bottles, when the examination is to be carried out within a short period after collection. While forwarding material in this preservative, it would be better to send larger pieces of the tissues, so that if necessary, cultures may be attempted from their central areas where the glycerine penetration has been the least.
Liquid material such as heart blood, cerebrospinal fluid and inflammatory exudates may be taken either on sterile swabs, sealed in pipettes or collected in tubes or bottles with strict aseptic precautions.
The surface of the organ should be well seared with a hot spatula and a sterile syringe and needle or the only helminth disease of humans spread by an insect vector is inserted into it for drawing the material. If the peritoneal fluid is required, an area on the abdominal wall is seared thoroughly and a sterile knife is used for holding the cavity open and sterile pipettes for drawing the fluid. For taking swabs of the contents of a closed abscess in a living animal, clip the hair from the area and paint it with tincture of iodine.
Open the abscess with a sterile scalpel and take swabs from the wall as well as from the contents of the abscess and keep the swabs in sterile tubes.
To collect wound discharge, the wound should be thoroughly cleaned with warm water and soap, and sterile non-antiseptic cotton wool or gauze dressing applied. The material should be collected after about 24 hours by inserting a sterile swab underneath the dressing.
For bacteriological examination of the intestinal flora, ligate about 6 inches of the bowel at both ends and forward the loop unopened under refrigeration. If there are abnormal contents in the uterus, a small segment is isolated between ligatures and retained unopened for bacteriological examination.
While it is desirable that the specimen for immunological study be refrigerated in transportation, serum for agglutination and complement fixation test may be preserved with 0. In swine, organisms are not present in blood, so swabs should be taken from exudates and the cut surface of hemorrhagic lymph nodes.
Flame fixed blood smears of cattle and sheep.
From subcutaneous swelling in horses, swine and dogs. Swabs of blood from ear vein for cultural examination from dead animals. It is not advisable to open the carcass suspected for anthrax in the field. If opened, it should be properly disposed by burning. Blackleg Black Quater : Impression smears from the affected muscle tissue; exudate from lesions; pieces of affected muscles on ice. Tetanus: Material from wound site isolation is not usually attempted Bacillary Haemoglobinuria: Affected liver tissue.
Botulism: Suspected food, meat, forage and urine. Enterotoxaemia, Lamb Dysentery: Intestinal pieces with contents inside tied with thread or contents of small intestine with and without chloroform separately on ice, kidney, urine.
Campylobacteriosis: Prepeutial washings, semen, foetal stomach contents, cervicovaginal mucus sample should reach the laboratory within 6 hours of collection under refrigeration or at room temperature in transport media. Brucellosis: Paired sera samples, blood and abomasal contents of aborted foetus, placenta with cotyledons, vaginal swabs in PBS, separate bottle on ice, whole foetus if small on ice. For ABR milk is used avoiding colustrum and milk from drying off animals or those suffering from mastitis.
Milk, serum, vaginal mucous etc.
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Haemorrhagic Septicemia: From sick animals fixed smears from blood and throat swelling and from dead animals, smears from heart blood and liver. Glanders: Exudate from skin and ung lesions in vials on ice.
Impression smears from exudate duly fixed, tissue containing early nodules, pus from ulcers. Salmonella Sp.
Paired sera samples. After collection, ectoparasites and intermediate hosts can be sent as such or after fixation. The chloroform is allowed to settle and the top solution is poured constituting the chloroform formalin mixture which retains the natural colour of ticks, if dropped alive.
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Fleas and lice are collected on a sheet of paper either by combing or with the help of a camel hair brush moistened with xylene.
Dead small birds and animals can be transported as such in securely tied polythene bags.
The ectoparasites can also be collected by dipping and washing the birds in a pail with water containing small amount of detergent. The parasites are collected with the help of a strainer.
Common blood protozoan parasites can be visualized in thin blood smears. For demonstration of microfilariae thick blood smears, whole blood or hemolysed with acetic acid centrifuged blood is desirable.
Вы ведь, кажется, сказали, что учились в университете. Беккер пожал плечами: - Наверное, в тот день я прогулял лекцию.
- Испанская церковь гордится тем, что ей принадлежат его останки.
The trophozoite forms of intestinal protozoa seldom survive in dead animal and at room temperature. Faeces or intestinal contents as such or in normal saline must, therefore, be examined immediately after collection or retained at body temperature till examined.
Protozoan cysts are comparatively resistant and can be identified in faeces for days. Refrigeration further preserves them. The sample of faeces to be cultured must be free from earthy or bedding contamination otherwise it may be heavily infected with free living nematodes ot their larvae. Theileriosis: 1 Biopsy smears from swollen lymph nodes from early stages of disease fixed with methanol. Blood smears fixed in methanol or alcohol.
Two to three blood smears from each case.
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Babesiosis: Thin blood smears from early stages of disease fixed with methanol. Anaplasmosis: Thin blood smears from ear vein fixed with methanol. Serological Samples: Paired samples one taken at the onset of the disease and another taken weeks later aree desirable.
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For anaplasmosis it should be frozen or preserved with 0. In other cases, it may be preserved with sodium merthiolate or carbolic acid Immunoflourescense Samples: Serum as such and freshly collected, refrigerated or frozen tissue. Biopsy Samples: 1. Bone marrow smears for hemopoietic disorders.
Intrebarea nr. Prisacaru CM Basic prevention measures in intestinal infections are: a [x] detection of infection sources b [x] detection and treatment of carriers c [x] sanitary-hygienic measures d [ ] vaccination of susceptible persons e [x] prophylactic and terminal disinfection Prisacaru CM Epidemiological classification of infectiuos diseases is based on: a [x] source of infection b [ ] clinical evolution of disease c [x] mechanism of transmission of infection d [ ] routes of transmission of infection e [x] ecological relationship with animals Prisacaru CM In case of habitual contact, for epidemic process are characteristic: a [x] appearance of diseases in chain b [ ] minimum period of incubation c [x] uniform contamination of all age groups d [ ] joining to the hydric factor e [x] HAV transmission, E.
Lymph nodes for lymphosarcoma whole or part 3. Liver biopsies for histopathology or chemical estimation 4. Blood Samples: 1. Blood smears for hemopoietic disorders or blood infections shluld be fixed in methyl alcohol, dried and sent. Blood samples for haematology-Anticoagulants viz. Anticoagulants for Blood: a. Heparin i. Oxalate phenol glycerine OCG solution-1 part to 2 parts of blood erythrocytes.
Blood is transported in chilled condition, but never frozen for both clinical examination as well as virus isolation. Where bacterial isolation is not required, antibiotics may be added. Cerebrospinal Fluid: Examination soon after collection is desirable. For leucocytes EDTA and for glucose estimation sodium fluoride is added as a preservative.
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Serous Cavity Fluid: No preservative is required and used for bacteriological or cytological examination. Sputum: No preservative is required and is used for bacteriological or parasitological examination. Toxicological Examination: In case suspected for poisoning, each specimen should be forwarded for laboratory examination in a separate wide mouth glass stoppeered bottle or a jar under refrigeration without the addition of a preservative.
The material submitted to a laboratory should include stomach with its contents after tying both ends; about 30 cm each of ileum and colon and their contents with their ends tied. In the case of ruminants, about 1 cancer genetic counselor salary of well mixed contents of rumen; about 0. In cases that may result in Vetero-legal action, particular care should be exercised for safe possesion from the time the specimens are collected until they are delivered to the toxicologist.
The type of poison suspected should be stated to assist in the laboratory diagnosis. All bottles and packings should be carefully sealed by the officer making the examination, closed in such a manner that they cannot be opened without destroying the seal.
When an officer forwards articles to the chemical examiner or toxicologist for examination, he should at the same time address and forward separately a letter to the chemical examiner regarding their dispatch, the letter should contain: 1.
An the only helminth disease of humans spread by an insect vector is of the seal used in closing 2. A list of articles forwarded and information as to how the articles have been forwarded. The name of the Officer from whom the order has been received to forward the articles, and the number and date of such order.
Information as to the number and kind of animals affected and the number of deaths. Any information obtained on post mortem examination, nature and duration of symptoms which may be likely to indicate the probable nature of the poison. Aflatoxicosis: 1 Suspected feed specially groundnut cake about g each 2 Piece of liver 50gspleen in formal saline and on ice separately Poisoning Cases: 1 Stomach and intestinal contents g on ice 2 Left over fodder g 3 About g liver pieces in alcohol on ice.
Poisoning: S. Suspected Poison Required Material In order of importance 1. Arsenic Acute 1. Liver 2. Kidneys 3. Stomach Contents 2.
Arsenic Chronic 1. Hair 2. Liver 3. Urine 3. Alkaloids 1. Brain 4. Stomach Contents 4. Copper 1. Liver 5. Cyanide 1. Oxalated Blood 6. Insecticides Chlorinated 1.
Microbiology of Helminths & Immunity
Fat 2. Lymphoid Organs 7. Insecticides Organophosphate 1. Oxalated Blood 2. Lymphoid Organs 8.